This project was carried out to determine the effect of poor teacher-pupil relationships on the academic achievement of Enugu South Local Government biology students. The literature was reviewed under the following subheading; the concept of a teacher, improving the academic achievement of the learner, factors affecting teacher–pupil relationship in the learning environment, the implication of poor teacher-pupil relationship in academic achievement and a summary of the review of related literature. This study was conducted in Enugu South Local Government, which is made up of nine (9) secondary schools. Out of the total number, five (5) were randomly sampled and used for this research work. Six (6) research questions guided the study. The instrument used to obtain information was questionnaire items drawn from six (6) research questions. The data was collected and analyzed using frequency tables and percentages. From the analysis of data, the following was found; that biology teachers use praises the pupils in their class, that biology teachers use leisure time to engage the pupils in a friendly chat, that biology teachers punish and react towards pupils in the class whenever they need their attention, that biology teachers allow pupils’ freedom, that students communicate freely with their biology teachers in the classroom and that relationship with biology teachers affect the performance of the students in the subject. Based on the findings, the researcher recommends that the government try to provide the necessary educational facilities and equipment to the schools and also employ biology teachers that possess the minimum qualification of Nigeria certification in education (NCE).
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